An Alternative to Toluidin Blue for Staining of EPON-Sections

Ruthenium Red Staining of Chromatin in Epon Sections

For a long time, the ammoniated ruthenium oxychloride “ruthenium red” (today formulated [1, 2] as [(NH3)5 R u •O • Ru (NH3)4 O • Ru (NH3)5]6+ • Clg), has been used as a microscopic test for pectins [2, 3]. Several applications of this contrasting agent for electron microscopic detection of acid polysaccharides and proteoglycans have been stud­ ied [4-10]. Its use as a Schiff-like reagent was re...

Somatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining

The confirmation of target expression in tissues is a prerequisite for molecular-targeted therapy. However, difficulties are sometimes associated with the production of appropriate antibodies against receptors. We herein developed a ligand derivative dye for the staining of receptors. The somatostatin receptor (sstr) was selected as the target and FITC-octreotate as the detective agent. We perf...

an application of fuzzy logic for car insurance underwriting

در ایران بیمه خودرو سهم بزرگی در صنعت بیمه دارد. تعیین حق بیمه مناسب و عادلانه نیازمند طبقه بندی خریداران بیمه نامه براساس خطرات احتمالی آنها است. عوامل ریسکی فراوانی می تواند بر این قیمت گذاری تاثیر بگذارد. طبقه بندی و تعیین میزان تاثیر گذاری هر عامل ریسکی بر قیمت گذاری بیمه خودرو پیچیدگی خاصی دارد. در این پایان نامه سعی در ارائه راهی جدید برای طبقه بندی عوامل ریسکی با استفاده از اصول و روش ها...

development and implementation of an optimized control strategy for induction machine in an electric vehicle

in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...

Non-nuclear staining of thick tissue sections.